Journal: Science immunology

Article Title: Human Innate Lymphoid Cell Precursors Express CD48 that Modulates ILC Differentiation through 2B4 Signaling

doi: 10.1126/sciimmunol.aay4218

Figure Lengend Snippet: (A) Freshly isolated UCB-derived CD34+ HSCs were stained for CD244 and CD48 (representative, n=4). (B) CD244 in CD34+CD48− cells (aqua) and in CD34+CD48+ cells (red) for day 1 and day 3 UCB-derived CD34+ HSCs (representative, n=4). (C) SAP mRNA expression in day 5 CD34+CD48− and CD34+CD48+ cells (n=4/group). (D and E) Anti-CD244 and anti-CD48 blocking antibodies or isotype IgG controls? were added to differentiating CD34+α4β7+CD48+ cultures to block CD244 signaling. (F and G) CD34+α4β7+CD48+ cells were plated on anti-CD244 or IgG coated plates twice for 4 days prior to 21 days of differentiation to activate CD244 signaling via cross-linking. Cells were stained for ILCs, and the percentage (D and F) or absolute number (E and G) of CD94+ NK cells, CD294+ ILC2 and CD117+ ILC3 are shown (n=4/group). (H) CD244+ cultures are shown for cells transfected with control or CD244 gRNA (n=3/group). (I) Representative ILC2 (CD294) staining for cultures transfected with control or CD244 gRNA. (J) Dot plot showing generation of ILC2s from cultures that express (control gRNA) or lack (CD244 gRNA) CD244. (C-H) Mean ± SD, paired t-tests (C, F, G and H) or one-way ANOVA (D and E) and p-values are depicted.

Article Snippet: The Alt-R Cas9 Nuclease V3, universal tracrRNA, CD244 gRNA and non-human control gRNA were purchased from Integrated DNA Technologies.

Techniques: Isolation, Derivative Assay, Staining, Expressing, Blocking Assay, Transfection

Journal: bioRxiv

Article Title: Detection of race-specific resistance against Puccinia coronata f. sp. avenae in Brachypodium species

doi: 10.1101/281568

Figure Lengend Snippet: Colonization of Brachypodium accessions by P. coronata f. sp. avenae . A . Fungal growth estimates in foliar tissue for isolates 12SD80, 203, and 12NC29 depicted by the percentage of colonized area at 14 dpi. Crown rust susceptible oat ( A. sativa ) variety Marvelous serves as positive experimental control. Results from two independent experiments for each isolate are shown with distinct color intensities and each bar represents a mean value in one independent experiment (biological replicate). Error bars represent standard error of mean of three leaves (technical replicates) within one independent experiment. B . Quantification of fungal DNA for each rust isolate in B. distachyon accessions ABR6 (blue line) and Bd21 (orange line). Error bars represent standard error of mean of four independent experiments (biological replicates).

Article Snippet: The relative abundance of fungal DNA was measured using the Femto™ Fungal DNA Quantification Kit (Zymo Research) based on quantification of ITS regions using ITS-specific primers and fungal DNA Standards provided by the manufacturer.

Techniques: